Title: Effect of hydroalcoholic extract of camellia sinensis on the viability and apoptosis of oral squamous cell carcinoma cell line
Abstract:
Background and aim: In recent years, green tea (Camellia Sinensis) has been evaluated in the treatment of different types of cancers due to its various antioxidants, fewer side effects, and lower toxicity. Many studies have shown that green tea has anti-proliferative and apoptosis-inducing effects. The aim of this study was to determine the effect of hydroalcoholic extract of green tea on the viability and apoptosis of oral squamous cell carcinoma cell line.
Methods: In this exprimental in-vitro study, oral squamous cell carcinoma (TSCC-1) IBRC C10555 and normal fibroblast cell lines (HuGu) IBRC C10459 were cultured in special plates and after producing green tea extract the cells were exposed to different concentrations of this substance (6.3, 12.5, 25 , 50, 100, 200, 400, 800, 1600 and 3200 μg/ml). Then, after 48h of exposure, viability of cells was checked by MTT assay and the rate of apoptotic cells were investigated by flow cytometry (Annexin V-FITC/PI kit) according to the instructions of the manufacturer. One Way ANOVA and Independent sample t-test were used to analyze the amount of living cells data, and One Way ANOVA and One-Sample Test were used to investigate apoptosis.
Results: The survival of human TSCC-1 cells decreased significantly with increasing concentration of green tea extract in a dose-dependent manner (p=0.000). As well, the survival of normal fibroblast (HuGu) cells decreased significantly with increasing concentration (p=0.000). Comparing the number of living cells between the two cell lines showed that viable SCC cells were significantly reduced compared to fibroblasts only at a concentration of 3200 µg/ml. In the fibroblast cell line, the amount of apoptotic and necrotic cells increased significantly with the increase in the concentration of the extract. However, in the oral squamous cell carcinoma cell line, the amount of apoptotic cells significantly decreased and the amount of necrotic cells increased significantly with the increase in the concentration of the extract (P<0.05).
Conclusion: Green tea extract was effective in decreasing survival of SCC cells and this effect was more visible at higher concentrations (3200 μg/ml) in killing TSCC-1 cells than HuGu cells.
Audience Take Away:
- The audience will learn the characteristics of plants and their use in the treatment of different diseases based on scientific research.
- This study will guide other audience to perform further studies on different substances.
- The use of medicinal plants in combination with chemical drugs will decrease the dose of chemical drugs and so prevents more side effects. But as I said before we should use these plants after scientific confirmation and under the supervision of clinicians.
