Abstract:
Recently, human natal dental pulp stem cells (hNDP-SCs) have been characterized in vitro and it has been shown that they satisfy criteria defining human mesenchymal stromal cells (MSCs), as proposed by the International Society for Cellular Therapy. However, these results were reached in the presence of xenogeneic expansion medium, which has the potential to alter the cells’ functional capacity. To determine the validity of the previously reported hNDP-SCs characteristics for human cell therapy, we have cultured hNDP-SCs in allogeneic expansion medium.Methods: Two hNDP-SC lineages were isolated from vital natal teeth, donated by a healthy newborn female and cultured in 2 % platelet rich plasma (PRP). Analysis of the phenotypic expressions, proliferation rates, viability, telomerase length and in vitro adipogenic, osteogenic and chondrogenic differentiation potentials of two hNDP-SCs lineages (Zn001 and Zn002) were performed. Results: both lineages displayed similar morphology, proliferation rates, adipogenic, chondrogenic and osteogenic differentiation potential. Telomere shortening by 41.0 % and 13.49% occurred from 3rd till 14th passage for lineages Zn001 and Zn002 respectively. Viability of both lineages was higher than 90 %. Flow cytometry demonstrated that both lineages were positive to the majority of tested markers, including markers, which were negatively, expressed when hNDP-SCs were cultured previously in xenogeneic medium. Using immune-cytochemistry the cells were shown to express beta III-tubulin, nestin, neurofilaments and Nanog. Conclusion: PRP used as allogeneic medium is a suitable cultivation medium for hNDP-SCs.
Biography:
Dr. Sherine Adel Nasry was born in Cairo, Egypt, in 1966. She received her education in the German School (DSB) in Egypt, and is fluent in Arabic, English, and German languages with fair knowledge in French. In 1989 she received her B.Sc. in Oral and Dental Medicine as well as the MSc and Ph. D degrees in Oral Diagnosis, Oral Medicine and Periodontology from Faculty of Dentistry, Cairo University, Egypt, in 2004 and 2008, respectively. In 2014 she was also awarded a Diploma in the Management of Technology from Nile University.
In July 2004, she joined the National Research Centre, Cairo, Egypt as an Assistant Researcher. Was promoted to Researcher in 2009 and to Associate Professor in 2014, and is currently holding the position of Ass. Prof. in the Surgery and Oral Medicine department, Oral and Dental Research Division, NRC, Egypt.
- Her current research interests and publications include Regenerative Medicine in the Oral Stem Cell Research Field as well as Oral Medicine and Diagnostic Research. Dr. Sherine is a member of The Egyptian Dental Syndicate, The Egyptian Dental Union, The International Association for Dental Research, The Arab Society of Medical Research, and the Technology, Innovation and Commercialization Office (TICO) in the National Research Centre
- Previous experience includes working as general practitioner at the Ministry of Health, a Lecturer at the Faculty of Oral Medicine and Periodontology in Misr University for Science and Technology (MUST). Dr. Sherine also participated as a Private Investigator (PI), Co PI and as a project member in several national projects and in several national and international stem cell workshops. Certificate of achievements received include certificate of outstanding performance in the research output in 2012 and 2013 in the NRC as well as several certificates of appreciation from the International Association for Dental Research and from the International Dental Congress in Cairo/Egypt.
