6th Edition of International Conference on Dentistry
and Oral Health

August 11-13, 2022

August 11-13, 2022 | Online Event
ICDO 2022

Extraction and purification of immunoglobulin y (igy)and assessment of antimicrobial efficacy of igy against dental pathogens..an Invitro study

Amitha HA, Speaker at ICDO-2022 dental conference
VS dental college and Hospital, India
Title: Extraction and purification of immunoglobulin y (igy)and assessment of antimicrobial efficacy of igy against dental pathogens..an Invitro study


Oral health and general wellbeing are inextricably bound and the eruption of teeth during firs year leads to colonization of Streptococcus Mutans (S.utans). Dental caries, an ever- growing public health problem is not much amenable to existing preventive measures. The aim of this study is to compare the imicrobial efficacy of the chicken egg yolk antibodies, Immunoglobulin (IgY), against Streptococcus Mutans (S. Mutans), Enterococcus Faecalis (E Faecalis)) and Lactobacillus on 1-,15- and 30-day intervals. IgY is extracted and purified by Polyethylene Glycol (PEG) precipitation method and the extracts are added to the culture plates containing S. Mutans, E. Faecalis and Lactobacillus by the well diffusion method and measurement of the diameter of the clear zone as an indication of bacterial growth inhibitory response by an antibacterial compound is done. Diameter of inhibition zones, which was clear area (there was no bacterial growth) were measured using a caliper. A repeated measures ANOVA with a Greenhouse-Geisser correction to determine the mean of S. Mutans, E. Faecalis and Lactobacillus between the time points show that there is tatistically significant result in the effect of IgY against Lactobacillus and E.Faecalis. There is antimicrobial effect of IgY against S. Mutans but the result is not statistically significant in between the time intervals. Results of this study demonstrate that IgY has antimicrobial effects against dental pathogens; S. Mutans, E. faecalis and Lactobacillus.


Dr. Amitha H.A(presently working as Associate Professor in the Department of Pediatric dentistry) completed her under graduation (B.D.S) from A.B Shetty Institute of Dental Science and MDS (Pediatric dentistry) from V.S Dental and Hospital (under RGUHS Bangalore). As an undergraduate, she was a top student in the subject of Community dentistry and Oral medicine and also securing second rank in conservative dentistry under RGUHS (2002-2007). As a post graduate, she secured 5th rank in pediatric Dentistry. As an Assistant and Associate Professor in Department of pediatric and preventive dentistry in V.S dental College and Hospital, she presented numerous poster and papers in various National and International Conferences with several national as well as international publications in her name. She has also undergone training in Nitrous oxide Inhalation management of Special Children