Title: Extraction and purification of immunoglobulin y (igy)and assessment of antimicrobial efficacy of igy against dental pathogens..an Invitro study
Oral health and general wellbeing are inextricably bound and the eruption of teeth during firs year leads to colonization of Streptococcus Mutans (S.utans). Dental caries, an ever- growing public health problem is not much amenable to existing preventive measures. The aim of this study is to compare the imicrobial efficacy of the chicken egg yolk antibodies, Immunoglobulin (IgY), against Streptococcus Mutans (S. Mutans), Enterococcus Faecalis (E Faecalis)) and Lactobacillus on 1-,15- and 30-day intervals. IgY is extracted and purified by Polyethylene Glycol (PEG) precipitation method and the extracts are added to the culture plates containing S. Mutans, E. Faecalis and Lactobacillus by the well diffusion method and measurement of the diameter of the clear zone as an indication of bacterial growth inhibitory response by an antibacterial compound is done. Diameter of inhibition zones, which was clear area (there was no bacterial growth) were measured using a caliper. A repeated measures ANOVA with a Greenhouse-Geisser correction to determine the mean of S. Mutans, E. Faecalis and Lactobacillus between the time points show that there is tatistically significant result in the effect of IgY against Lactobacillus and E.Faecalis. There is antimicrobial effect of IgY against S. Mutans but the result is not statistically significant in between the time intervals. Results of this study demonstrate that IgY has antimicrobial effects against dental pathogens; S. Mutans, E. faecalis and Lactobacillus.