Speaker for dentistry conferences 2019 - Susanne Stahlke

Title: Complex cell physiology on chemically and topographically designed implant surfaces

Susanne Staehlke

University of Rostock, Rostock University Medical Center, Germany

Biography

Susanne Staehlke studied Genetic and Microbiology at the University of Rostock, Germany, from 2000-2006. The subject of her diploma was signal transduction in rainbow trout (Oncorhynchus mykiss). In 2014, she earned her Ph.D. in Cell Biology at Rostock University Medical Center, Dept. of Cell Biology, Germany, with the title “Interaction of human osteoblasts with geometrically structured implant surfaces – Cell architecture and signal transduction”. Now she is a young researcher completing a post-doctoral training. She is a Member of the German Society for Biomaterials (DGBM). Dr. Staehlke has published over 15 papers on the subject of cell-material interactions. Her skills are cell culture, physical plasma, western blot, immunofluorescence, biomedical science, flow cytometry, cell proliferation, cell signaling and transduction. Dr. Staehlke has given invited presentations at international meetings around the world (Hong Kong: ICBB 2012, Minneapolis: BioInterface 2013, Oslo: ScSB 2013, Rytro: PSBM 2016-2018).

Abstract

Implant biomaterials should be bioactive in stimulating the surrounding tissue to accelerate the ingrowth of permanent implants, e.g. the dental implants. The critical factor for the ingrowth of permanent implants into bone tissue is rapid cellular acceptance. Although the surface topography is a decisive factor for cell function and adhesion, it turns out that a specific surface chemistry with positive charge carriers can dominate over the topography. Recently, we have shown that a plasma-chemical modification using allylamine as precursor was able to boost not only cell attachment, spreading and cell migration but also intracellular signaling in vital cells. The calcium ion mobilisation capacity was decisively higher in cells growing on plasma polymerised allylamine (PPAAm). The PPAAm plasma process generated a homogenous nanolayer (~50 nm) with randomly distributed, positively charged amino groups. The membrane surface of human osteoblasts is negatively charged due to their spherical coat composed of the extracellular matrix component hyaluronan. Interestingly, sharp-edged micro topographies can be enwrapped completely by cells if coated with the nanolayer of PPAAm. In addition, cells are able to overcome the restrictions of micro-grooves, i.e. the topographically induced contact guidance. However, coating with collagen type I or immobilisation of the integrin adhesion peptide sequence Arg-Gly-Asp (RGD) could not induce the same effect. This plasma-chemical nanocoating PPAAm can be used for several biomaterials in orthopaedic and dental implantology, e.g. titanium, titanium alloys, calcium phosphate scaffolds, or ceramics using Yttria-stabilised tetragonal zirconia [Staehlke S et al. Cell & Bioscience 2018; Moerke C et al. ACS Applied Material Interfaces 2017; Moerke C et al. Biomaterials 2016; Staehlke S et al. Biomaterials 2015; Finke B et al. Biomaterials 2007].