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Sayedah Zahra Rahmani

potential speaker for international dental conferences 2017 - Sayedah Zahra Rahmani

Title: Evaluation of in vitro biofilm removal with 2% and 10% sodium hypochlorite

Sayedah Zahra Rahmani

Behbood Hospital, Iran

Biography

I was born in 1991 in Tabriz Iran 
I have finished my high school and college  in high talented students school(SAMPAD) in 2008
I earned scholarship for dental school of Tabriz university of medical sciences and graduated top 10 in my class on 2015 and also i was the only dentistry student won the  international research fellowship in Groningen Netherlands  in 2015 
I started working in Behbood hospital as general dentist in 2015 till now
I am interested in research in medical and also basic science fields as well as dental practice and i wish to continue my studies as an post graduate student and specialize in one of the fields of my interest and maybe become a professor to continue research and gaining and training dental knowledge.

Abstract

Introduction
Biofilms are communities of microorganisms attached to a surface and embedded in a matrix of polysaccharides and proteins forming a slimy layer. Oral bacteria have the capacity to form biofilms on distinct surfaces. Bacteria also form dense colonies on root canal walls and features like isthmusses and lateral canals. Microbial communities in biofilms are remarkably difficult to eradicate with antimicrobial agents for reasons that have yet to be adequately explained.
Studies have shown that sodium hypochlorite (NaOCl) is the most effective anti-microbial irrigant used during endodontic treatment. The aim of this study is to evaluate the structure of biofilms and presence of EPS before and after the use of NaOCl 2% and 10%.
 
Materials and methods
Dual species biofilms of Streptococcus oralis J22 and Actinomyces naeslundii T14VJ1 were grown under statical conditions and in a constant depth film fermentor (CDFF). Biofilms grown in the CDFF mimic better the basal layer of an oral in vivo biofilm. For the statical conditions, a confined space was created over saliva coated dentin discs with supply of 20ml of modified BHI each 24h for 4 and 10 days. For the CDFF, saliva coated hydroxyapatite discs biofilm was grown for 96 h at 37uC under continuous supply modified BHI at a rate of 45 ml/h. The system was equipped with 15 sample holders and each sample holder contained 5 saliva coated hydroxyapatite discs, recessed to a depth of 250 mm. After growing the biofilms NaOCl 2% and 10% were applied for 60s and 300s for removing the biofilm. Optical coherence tomography (OCT) was used for high-resolution, real-time imaging of a three-dimensional structure of the biofilm. Confocal laser scanning microscopy (CLSM) was used to vizualise the biofilm matrix, structure and condition of bacteria (LIVE/DEAD staining).
 
Results
In the statical biofilm group, OCT images showed reduction of biofilm thickness after applying the NaOCl 2% and 10% and there was a very fluffy structure observable. In the CDFF group, OCT images showed bubble formation in the biofilm after using NaOCl 10%, but the irrigant did not reduce the thickness of the biofilm or on its consistency. The bubble formation was also observed in CLSM images. The CLSM showed reduction of the biofilm structure but mostly living bacteria were found in the remaining biofilm.

Conclusion

Due to our study our simple irrigation methods are not efficient enough for biofilm removal and we suggest to use irrigants in several times with increased applying time to achieve better biofilm removal and better treatment results.